Working with Odorous Noxious Substances

Please note the following chemicals generating noxious or unpleasant odors and their appropriate use in DMCBH facilities.

The list below is not comprehensive. If you have questions or concerns about noxious substance use, please contact your lab manager.

TEMED/Tetramethylethylenediamine 

  • No maximum working concentration in lab.
  • Keep the stock bottles open for the shortest amount of time possible, please seal immediately after the contents are used.
  • Ensure usage is in well-ventilated area. Sealed during transport.
  • Bench-top tip receptacles are to have lids are to be emptied daily. 

Bleach

  • Maximum working concentration in open lab: 10 per cent. Concentrations over 10 per cent are to be used in fume hoods.
  • Bottles of bleach can be open only during the preparation of solutions. Sealed during transport
  • Disposal: drain and flush with copious amounts of water, or in a sealed container.  

2-Mercaptoethanol

Maximum working concentration in open lab: 10 per cent or less. Concentrations over 10 per cent are to be used in fume hoods

  • The stock bottle and other forms must be sealed when transported.
  • Tubes must be sealed during denaturing preparation for western blots.
  • Loading buffers or other preparations with similar working concentrations of 2-mercaptoethanol (10 per cent), may be used in the open lab areas.
  • Warning: High concentration of 2-Mercaptoethanol vapors can cause respiratory distress.